oYo-Capture™ Magnetic Beads

For the rapid removal of free oYo-Link after antibody conjugation to ensure low background in high sensitivity assays.

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oYo-CaptureTM Magnetic Beads enable the efficient removal of free oYo-Link after antibody conjugation to ensure low background in high sensitivity assays. oYo-Capture enables the purification of up to 1mg of conjugated antibody.

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For Research Use Only.

oYo-Capture™ Magnetic beads provide a an efficient method of removing free oYo-Link® reagents post antibody conjugation to ensure low background in high sensitivity assays.

oYo-Link technology enables highly efficient labeling reactions, resulting in only a small excess of free oYo-Link post conjugation. However, for researchers carrying out highly sensitive assays, oYo-Capture enables the quick and easy removal of unconjugated oYo-Link in only 5 minutes, providing reassurance that there will be low assay background. Furthermore, oYo-Capture ensures excellent recovery of materials, so there is no risk of wasting precious antibody conjugate in the purification process.

Requirements: To use oYo-Capture Magnetic Beads you will need a magnetic stand.

Purchasing Information: For credit card purchases, proceed with checkout on website. Or request a quote here.


Technical Support: For technical questions contact us here.

Antibody-oYo-Link® Conjugate Purification Protocol

This protocol describes how to remove free oYo-Link from conjugated antibody-oYo-Link using oYo-Capture Magnetic beads.

oYo-Link® Product Brochure

Download our product brochure

Free oYo-Link removal with oYo-Capture

Figure 1. Shows the absence of oYo-Link Azide-TAMRA after removal with oYo-Capture for only 5 minutes.

oYo-Capture Magnetic Beads Antibody Recovery Data.

Figure 2. oYo-Capture Magnetic Beads Antibody Recovery Data. 25 ug human IgG1-oYo-Link was mixed and captured by 100 uL of oYo-Capture beads (10 mg/mL concentration). As shown on the gel, the antibody heavy chain remains largely unchanged after purification (Lane 4 to Lane 5), indicating excellent recovery of materials.

Catalog Amount Labeled Price(USD) Spec Sheet
AT8101-10 Purifies up to 1mg of conjugated antibody $350 Download Here

Specifications:

Product name

oYo-Capture™ Magnetic Beads

Catalog #

AT8101-10

Lot #

See the product spec sheet shipped with product.

MSDS

Download the MSDS here.

Beads Coating

Rabbit IgG

Beads Concentration

10 mg/mL

Beads Volume

4 mL

Storage Buffer

1xPBS + 0.1% Sodium Azide

Shipping Condition

The product is shipped as brown liquid at ambient temperature.

Storage and Stability

The product can be stored at 4oC for one year from date shipped.

Notes

Disposal of product should be performed in compliance with all applicable federal, state, and local regulations.
This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

  • Ensure low background in high sensitivity assays
  • Rapid removal free oYo-Link in only 5 minutes
  • Ensures excellent recovery of antibody conjugates